Part:BBa_K1648046
Mutated AvOprF
OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode.
According to a paper concerning the factors affecting the conformation of OprF, we found that mutation on four Cysteine to four Serine and Lysine to Glycine at 189th position (K189G; C201S; C210S; C216S; C230S) of Azotobacter vinelandii OprF will give higher possibility of open-channel conformation with high porin activity by 5 folds. With the insertion of this mutated OprF into the bacteria, the possibly of electron carrier transmission between bacteria and extracellular as well as the efficiency of MFC would be increased by five folds. BBa_K1648046 is the basic part including RBS, mutated oprF and R0040(double terminator).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 495
Illegal BsaI site found at 617
| n/a | Mutated AvOprF |